Identification of storage-protein messenger RNA of the fleshfly Sarcophaga peregrina

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Identification of storage-protein messenger RNA of the fleshfly Sarcophaga peregrina.

Storage-protein mRNA was found to be abundant in poly(A)-containing RNA extracted from the fat-body of third-instar larvae of Sarcophaga peregrina (fleshfly). This RNA sedimented at the position of 19S on sucrose-density-gradient centrifugation and the product of its translation in vitro was 75K protein (protein of mol.wt. 75 000), which was precipitated specifically with antibody against stora...

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We chose the larvae of fleshfly Sarcophaga bullata to map the peptide and protein immune response. The hemolymph of the third-instar larvae of S. bullata was used for isolation. The larvae were injected with bacterial suspension to induce an antimicrobial response. The hemolymph was separated into crude fractions, which were subdivided by RP-HPLC, gel electrophoresis, and free-flow electrophore...

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Sugar receptor specificity in the fleshfly, Sarcophaga bullata.

The sugar receptor of insects is a useful preparation for studies of receptor specificity compared to the more complex sensors of vertebrates. Unlike the vertebrates, insects appear to respond behaviorally only to carbohydrates. Von Frisch v and Dethier a using the honey bee, Apis mellifera, and the blowfly, Phormia regina, respectively, were able to make inferences concerning a common structur...

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Ionophore activity of sarcotoxin I, a bactericidal protein of Sarcophaga peregrina.

When Escherichia coli was treated with sarcotoxin I, a potent bactericidal protein of Sarcophaga peregrina (fleshfly), K+ inside of the cells leaked out rapidly and the ATP pool of the cells rapidly decreased. These results suggested that the bactericidal effect of sarcotoxin I was due to its ionophore activity, and that it blocked the generation of ATP by inhibiting formation of the proton gra...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1982

ISSN: 0264-6021

DOI: 10.1042/bj2030571